http://hdl.handle.net/11089/1305 2026-06-14T20:43:10Z 2026-06-14T20:43:10Z Mietlińska-Sauter, Joanna Battaglia, Sebastiano Lorkiewicz, Wieslaw Fruciano, Carmelo http://hdl.handle.net/11089/58462 2026-06-11T09:31:05Z 2024-06-26T00:00:00Z

Morphological structure of the vertebra and predisposition to fatigue spondylolysis in the human population - geometric morphometrics studies on skeletal material - MINIATURA 8 (2024/08/X/NZ8/00212) dataset Mietlińska-Sauter, Joanna; Battaglia, Sebastiano; Lorkiewicz, Wieslaw; Fruciano, Carmelo Mietlińska-Sauter, Joanna Fatigue spondylolysis is commonly interpreted in bioarchaeology as a direct skeletal marker of increased axial loading, particularly in pre-industrial populations engaged in strenuous manual labour. However, clinical studies in contemporary populations suggest that vertebral morphology may also play an important role in fracture susceptibility. The present project examined whether anatomical predisposition contributed to fatigue spondylolysis in historical human populations from the Brześć Kujawski microregion (Kuyavia, central Poland), dating from the early Middle Ages to the mid-nineteenth century. Fifth lumbar vertebrae (L5) representing individuals with and without spondylolytic fractures were selected from osteological collections housed at the University of Lodz. Following cleaning and, where necessary, recomposition, the vertebrae were scanned using structured-light 3D surface scanning. Their morphology was analysed using landmark-based geometric morphometrics, including fixed landmarks and sliding semilandmarks, and the resulting data were statistically evaluated in MorphoJ, R software environment and Statistica. The analyses revealed statistically significant differences in L5 anatomy between spondylolytic and non-pathological vertebrae. Fractured vertebrae displayed morphological features suggestive of functional predisposition to injury. In addition, previously undescribed differences in fluctuating asymmetry were identified between the two groups, indicating that developmental instability may also have contributed to fracture susceptibility. These findings suggest that fatigue spondylolysis in pre-industrial populations should not be regarded solely as a simple indicator of increased spinal loading. Instead, its occurrence appears to reflect an interaction between mechanical stress as well as individual anatomical and developmental predispositions. The study demonstrates the utility of geometric morphometrics for investigating vertebral variation in archaeological populations and provides a more nuanced framework for interpreting spondylolysis in bioarchaeological research. 1) Folder 1: representative scans in .STL format (one non-pathological vertebra, SBK4_59_L5, and one vertebra affected by fatigue spondylolysis, SBK4_250_L5) 2) Folder 2: reference landmark configuration used in the study (including a .vtk file containing the vertebral model, 26 .mrk.json files with landmarks assigned to individual landmark sets, an Excel file defining all landmarks, and a readme.txt file with instructions for opening the files) 3) Folder 3: digitization results for all 36 analysed vertebrae - 10 affected by spondylolysis and 26 controls (including two folders corresponding to the first and second digitization rounds, respectively; each folder contains files in four formats: .mrk.json, .json, .fcsv, and .csv). The folder also includes an Excel file defining all landmarks, presented without subdivision into individual vertebral elements, but distinguished as fixed landmarks and sliding semilandmarks on curves and surfaces. 4) Folder 4: output models (including two .ply files representing the mean shape of a spondylolytic vertebra and the mean shape of a non-pathological vertebra and a readme.txt file with instructions for opening the files)

2024-06-26T00:00:00Z Liudvytska, Oleksandra http://hdl.handle.net/11089/57929 2026-05-21T12:01:23Z 2026-03-31T00:00:00Z

Wstępna ocena potencjału terapeutycznego miechunki pomidorowej (Physalis philadelphica) w kontekście przebudowy tkanek i gojenia ran (dataset) Liudvytska, Oleksandra Chronic wounds represent a growing problem in lifestyle diseases (e.g., atherosclerosis, diabetes). They are characterized, among other things, by disturbances in the mechanisms controlling the course of inflammatory processes, degeneration of the tissue surrounding the wound, scarring, and susceptibility to infections. Hard‑to‑heal wounds constitute a serious and burdensome issue for patients, as they significantly reduce their quality of life and require long-term, specialized therapy. The search for natural bioactive substances is one of the important directions in contemporary wound‑healing research. Natural compounds can serve as the basis for therapies that support all stages of healing, including modulation of the inflammatory response, fibroblast proliferation, and epithelial regeneration. They are often characterized by high biocompatibility and low cytotoxicity, making them very promising candidates for applications in wound‑healing support. The project focused on key aspects relevant to the potential use of extracts from the tomatillo (Physalis philadelphica) in wound‑healing support: anti‑inflammatory properties, effects on tissue remodeling, assessment of cytotoxicity risk, and metabolism by the skin microbiome. Four extracts were tested: from fruits (green – unripe, and yellow), leaves, and roots. The study employed dermal (fibroblast) and epithelial (keratinocyte) cell lines. Additionally, the effects of the extracts on proteins of the fibrinolytic system were evaluated, as this system plays an important regulatory role in tissue remodeling within the wound area. Spis plików: 1. Generowanie plazminy 2. IL- 6 3. IL- 8 4. Mikrobiota skóry 5. MMP3 6. MMP9 7. MTT 8. PLUA 9. PLUAR soluble 10. PLUAR 11. Test rany

2026-03-31T00:00:00Z Soboska, Kamila http://hdl.handle.net/11089/57731 2026-03-19T03:18:39Z 2024-09-01T00:00:00Z

Opracowanie modelu badawczego in vitro do analiz wpływu stanu ketozy na angiogenezę nowotworową; Miniatura 8 (2024/08/X/NZ3/00599) (dataset) Soboska, Kamila As part of the Miniatura 8 research project, the effect of the ketosis state on tumour-associated angiogenesis was investigated. An in vitro model reflecting the conditions during ketogenic diet was developed, and the effects of glucose deprivation and ketone bodies were compared in tumour (TECs) and normal (NECs) endothelial cells. The studies were performed using the HMEC-1 cell line, in which a tumour endothelial cell model was generated under hypoxic conditions and in the presence of cancer cell-secreted pro-angiogenic factors. The expression of ketone body transporters and ketolytic enzymes was analysed at both the mRNA and protein levels in NECs and TECs, and their ability to metabolise ketone bodies was compared. In further experiments, the effect of ketone bodies on endothelial cell viability and the secretion of pro-angiogenic factors by colorectal cancer cell lines were evaluated. The dataset contains the results of the following tests: sqPCR from the analysis of the expression of markers confirming tumor endothelial cell model (TECs markers_sqPCR_RawData) and from the analysis of the expression of genes encoding ketolytic enzymes and the ketone body transporter (ketolysis_sqPCR_RawData), images of the results of the Western immunoblot analysis (WB scan), acetyl-CoA concentration in cells treated with ketone bodies (Acetyl-CoA conc_RawData), results of endothelial cell proliferation tests under conditions of variable glucose concentration (ECs prolif_glucose conc_RawData) and in conditions of simulated ketosis (ECs prolif_KBs conc_RawData) and results of the analysis of cytokine profiling secreted by tumor cells under simulated ketosis conditions (ProteomeProfiler)

2024-09-01T00:00:00Z Gonciarz, Weronika http://hdl.handle.net/11089/57553 2026-05-21T12:11:00Z 2026-01-01T00:00:00Z

Untargeted metabolomic profiling for identifying systemic signatures of Helicobacter pylori infection in children (dataset) Gonciarz, Weronika Background. Infections caused by the Gram-negative bacterium Helicobacter pylori (H. pylori) can result in gastritis, gastric or duodenal ulcers, and gastric cancer in humans. Examining quantitative changes in soluble biomarkers linked to H. pylori infection offers a promising approach to monitor the infection's progression, inflammatory response, and systemic effects. Aim. This study aimed to identify specific metabolomic biomarkers in the sera from children infected with H. pylori. Materials and Methods. Sera from 32 H. pylori-infected children – Hp (+) and 32 uninfected children – Hp (-), under the pediatric gastroenterological medical care were used in this study. The H. pylori status in children was confirmed or excluded by gastroscopy, 13C urea breath testing, and the presence of serum anti-H. pylori IgG antibodies. Metabolomic profiling was performed using UPLC-QTOF/MS methods. Biomarkers significantly associated with H. pylori infection were identified using volcano plots and ROC analysis. Results. The study found 8 unique metabolites well differentiating the serum samples of children infected with H. pylori from children uninfected with these bacteria: carboxyethyl lysine - CEL (HMDB29447), gamma-Glutamylleucine (HMDB0011171), 13-HOTrE(y) hydroxylated and oxidized derivative of the omega-6 fatty acid arachidonic acid (HMDB0341541), 13-HODE (HMDB0004667), lauroylcarnitine (HMDB0002250), phosphatidylethanolamine (HMDB0060501), vitamin A (HMDB0000305) and 19_norandrosterone (HMDB0002697). These metabolites are associated with immune regulation, energy metabolism, lipid/fatty acid metabolism, lipid peroxidation, oxidative stress, and cell signaling, and correlate with the pathogenesis of H. pylori infection in humans. Conclusions. Serum metabolomic profiling combined with ROC analysis used in this study successfully differentiated H. pylori-infected from uninfected children. Further study is needed to link these selected metabolic biomarkers to the course of infection in children and to systemic effects of chronic infection.

2026-01-01T00:00:00Z