Pokaż uproszczony rekord

dc.contributor.authorGóralski, Paweł
dc.contributor.authorRogalinska, Malgorzata
dc.contributor.authorBłoński, Jerzy Z.
dc.contributor.authorPytel, Edyta
dc.contributor.authorRobak, Tadeusz
dc.contributor.authorKiliańska, Zofia M.
dc.contributor.authorPiekarski, Henryk
dc.date.accessioned2016-03-23T12:12:23Z
dc.date.available2016-03-23T12:12:23Z
dc.date.issued2014
dc.identifier.issn1388-6150
dc.identifier.urihttp://hdl.handle.net/11089/17526
dc.description.abstractChronic lymphocytic leukemia (CLL) is a heterogenous disease with an imbalance between apoptosis and cell proliferation. Therefore, the main goal in CLL therapy is to induce apoptosis and effectively support this process in transformed B lymphocytes. In the current study, we have compared differential scanning calorimetry (DSC) profiles of nuclei isolated from CLL cells and normal mononuclear cells exposed to cladribine or fludarabine combined with mafosfamide (CM; FM), and additionally to CM combined with monoclonal antibody—rituximab (RCM) for 48 h, as well as in culture medium only (controls). Under current study, the mononuclear cells from peripheral blood (PBMCs) of healthy individuals have been included. The obtained results have shown the presence of thermal transition at 95 ± 5 °C in most of nuclear preparations (92.2 %) isolated from blood of CLL patients. This thermal characteristic parameter was changed after drug exposure, however, to a different extent. These thermal changes were accompanied by the decrease of cell viability, an elevation of apoptosis rate and the changes in expression/proteolysis of poly(ADP-ribose)polymerase-1—main marker of apoptosis. Importantly, in DSC profiles of nuclear preparations of PBMCs from blood of healthy donors exposed to investigated drug combinations and control CLL cells, the lack of such changes was observed. Our results confirmed that DSC technique complemented with other biological approaches could be helpful in tailoring therapy for CLL patients.pl_PL
dc.description.sponsorshipResearch was sponsored by Grant from the Polish National Science Centre (No. 2011/01/B/NZ/0102); Results of presented study were partially presented in oral presentation on 2nd Central and Eastern European Conference on Thermal Analysis and Calorimetry in Vilnius, Lithuania, 2013pl_PL
dc.language.isoenpl_PL
dc.publisherSpringer Netherlandspl_PL
dc.relation.ispartofseriesJournal of Thermal Analysis and Calorimetry;2
dc.rightsUznanie autorstwa 3.0 Polska*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/pl/*
dc.subjectChronic lymphocytic leukemiapl_PL
dc.subjectDifferential scanning calorimetrypl_PL
dc.subjectAnti-leukemic tailored therapypl_PL
dc.subjectPurine analogspl_PL
dc.subjectMafosfamidepl_PL
dc.subjectRituximabpl_PL
dc.titleThe differences in thermal profiles between normal and leukemic cells exposed to anticancer drug evaluated by differential scanning calorimetrypl_PL
dc.typeArticlepl_PL
dc.page.number1339-1344pl_PL
dc.contributor.authorAffiliationUniversity of Lodz, Department of Physical Chemistrypl_PL
dc.contributor.authorAffiliationUniversity of Lodz, Department of Cytobiochemistrypl_PL
dc.contributor.authorAffiliationMedical University of Lodz, Department of Hematologypl_PL
dc.identifier.eissn1588-2926
dc.referencesScielzo C, Hacken ET, Bertilaccio MTS, Muzio M, Calissano C, Ghia P, Caligaris-Cappio F. How the microenvironment shapes chronic lymphocytic leukemia: the cytoskeleton connection. Leuk Lymph. 2010;51:1371–4pl_PL
dc.referencesTong W-G, Wierda WG, Lin E, Kuang SQ, Bekele BN, Estrov Z, Wei Y, Yang H, Keating MJ, Garcia-Manero G. Genome-wide DNA methylation profiling of chronic lymphocytic leukemia allows identification of epigenetically repressed molecular pathways with clinical impact. Epigenetics. 2010;5:499–508pl_PL
dc.referencesLongo PG, Laurenti L, Gobessi S, Sica S, Leone G, Efremov DG. The Akt/Mcl-1 pathway plays a prominent role in mediating antiapoptotic signals downstream of the B-cell receptor in chronic lymphocytic leukemia B cells. Blood. 2008;111:846–55pl_PL
dc.referencesKlein U, Dalla-Favera R. Germinal centres: role in B-cell physiology and malignancy. Nat Rev Immunol. 2008;8:22–33pl_PL
dc.referencesVeliz M, Pinilla-Ibarz J. Treatment of relapsed or refractory chronic lymphocytic leukemia. Cancer Control. 2012;19:37–53pl_PL
dc.referencesChen R, Plunkett W. Strategy to induce apoptosis and circumvent resistance in chronic lymphocytic leukemia. Best Pract Res Clin Haematol. 2010;23:155–66pl_PL
dc.referencesRassenti LZ, Jain S, Keating MJ, Wierda WG, Grever MR, Byrd JC, Kay NE, Brown JR, Gribben JG, Neuberg DS, He F, Greaves AW, Rai KR, Kipps TJ. Relative value of ZAP-70, CD38, and immunoglobulin mutation status in predicting aggressive disease in chronic lymphocytic leukemia. Blood. 2008;112:1923–30pl_PL
dc.referencesShanafelt TD, Rabe KG, Kay NE, Zent CS, Jelinek DF, Reinalda MS, Schwager SM, Bowen DA, Slager SL, Hanson CA, Call TG. Age at diagnosis and the utility of prognostic testing in patients with chronic lymphocytic leukemia. Cancer. 2010;116:4777–87pl_PL
dc.referencesFerrajoli A, Shanafelt TD, Ivan C, Shimizu M, Rabe KG, Nouraee N, Ikuo M, Ghosh AK, Lerner S, Rassenti LZ, Xiao L, Hu J, Reuben JM, Calin S, You MJ, Manning JT, Wierda WG, Estrov Z, O’Brien S, Kipps TJ, Keating MJ, Kay NE, Calin GA. Prognostic value of miR-155 in individuals with monoclonal B-cell lymphocytosis and patients with B-cell chronic leukemia. Blood. 2013;122:1891–9pl_PL
dc.referencesGarbett NC, Miller JJ, Jenson AB, Chaires JB. Calorimetric outside the box: a new window into the plasma proteome. Biophys J. 2008;94:1377–83pl_PL
dc.referencesMichnik A, Drzazga Z, Michalik K, Barczyk A, Santura I, Sozańska E, Pierzchała W. Differential scanning calorimetry study of blood serum in chronic obstructive pulmonary disease. J Therm Anal Calorim. 2010;102:57–60pl_PL
dc.referencesFekecs T, Zapf I, Ferencz A, Lorniczy D. Differential scanning calorimetry (DSC) analysis of human plasma in melanoma patients with or without regional lymph node metastases. J Therm Anal Calorim. 2012;108:149–52pl_PL
dc.referencesPili B, Bourgaux C, Meneau F, Couvreur P, Ollivon M. Interaction of anticancer drug gemcitabine with phospholipid bilayers. J Therm Anal Calorim. 2009;98:19–28pl_PL
dc.referencesSturtevant JM. Biochemical applications of differential scanning calorimetry. Ann Rev Phys Chem. 1987;38:463–88pl_PL
dc.referencesAlmagor M, Cole RD. Differential scanning calorimetry of nuclei as a test for the effects of anticancer drugs on human chromatin. Cancer Res. 1989;49:5561–6pl_PL
dc.referencesBalbi C, Abelmoschi ML, Gogioso L, Parodi S, Barboro P, Cavazza B, Patrone E. Structural domains and conformational changes in nuclear chromatin: a quantitative thermodynamic approach by differential scanning calorimetry. Biochemistry. 1989;28:3220–7pl_PL
dc.referencesAllera C, Lazzarini G, Patrone E, Alberti I, Barboro P, Sanna P, Melchiori A, Parodi S, Balbi C. J Biol Chem. 1997;272:10817–22pl_PL
dc.referencesRogalińska M, Góralski P, Kobylińska A, Błoński JZ, Hanausek M, Walaszek Z, Piekarski H, Robak T, Kiliańska ZM. Changes in leukemic cell nuclei revealed by differential scanning calorimetry. Leuk Lymph. 2005;46:121–8pl_PL
dc.referencesRogalinska M, Goralski P, Wozniak K, Bednarek JD, Blonski JZ, Robak T, Piekarski H, Hanausek M, Walaszek Z, Kilianska ZM. Calorimetric study as a potential test for choosing treatment of B-cell chronic lymphocytic leukemia. Leuk Res. 2009;33:308–14pl_PL
dc.referencesFurlan A, Villanova F, Pietrogrande F, Caladin M, Sanzari M, Vianello F. Low-dose fludarabine increases rituximab cytotoxicity in B-CLL cells by triggering caspases activation in vitro. Leuk Lymph. 2010;51:107–13pl_PL
dc.referencesChen R, Plunkett W. Strategy to induce apoptosis and circumvent resistance in chronic lymphocytic leukemia. Best Pract Res Clin Haematol. 2010;23:155–66pl_PL
dc.referencesRogalińska M, Błoński JZ, Komina O, Góralski P, Żołnierczyk JD, Piekarski H, Robak T, Kiliańska ZM, Węsierska-Gądek J. R-roscovitine (Seliciclib) affects CLL cells more strongly than combinations of fludarabine or cladribine with cyclophosphamide: inhibition of CDK7 sensitizes leukemic cells to caspase-dependent apoptosis. J Cell Biochem. 2010;109:217–35pl_PL
dc.referencesSabbah R, Xu-Wu A, Chickos JS, Planas Leitão ML, Roux MV, Torres LA. Reference materials for calorimetry and differential thermal analysis. Thermochim Acta. 1999;331:93–204pl_PL
dc.referencesRogalińska M, Franiak-Pietryga I, Błoński JZ, Góralski P, Maciejewski H, Janus A, Robak P, Mirowski M, Piekarski H, Robak T, Kiliańska ZM. Toward personalized therapy for chronic lymphocytic leukemia: DSC and cDNA microarray assessment of two cases. Canc Biol Ther. 2013;14:1–7pl_PL
dc.referencesRobak T, Smolewski P, Cebula B, Szmigielska-Kaplon A, Chojnowski K, Blonski JZ. Rituximab combined with cladribine or with cladribine and cyclophosphamide in heavily pretreated patients with indolent lymphoproliferative disorders and mantle cell lymphoma. Cancer. 2006;107:1542–50pl_PL
dc.referencesGarbett NC, Mekmaysy CS, Helm CW, Jenson AB, Chaires JB. Differential scanning calorimetry of blood plasma for clinical diagnosis and monitoring. Exp Mol Pathol. 2009;86:186–91pl_PL
dc.contributor.authorEmailkchfpiek@uni.lodz.plpl_PL
dc.identifier.doi10.1007/s10973-014-3957-2
dc.relation.volume118pl_PL


Pliki tej pozycji

Thumbnail
Thumbnail

Pozycja umieszczona jest w następujących kolekcjach

Pokaż uproszczony rekord

Uznanie autorstwa 3.0 Polska
Poza zaznaczonymi wyjątkami, licencja tej pozycji opisana jest jako Uznanie autorstwa 3.0 Polska