Melittin—A Natural Peptide from Bee Venom Which Induces Apoptosis in Human Leukaemia Cells
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Data
2020Autor
Gorniak, Leslaw
Ceremuga, Michał
Stela, Maksymilian
Janik, Edyta
Śliwiński, Tomasz
Sitarek, Przemysław
Saluk-Bijak, Joanna
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: Bee venom is a very complex mixture produced and secreted by the honeybee (Apis mellifera).
Melittin is a major component of bee venom that accounts for about 52% of its dry mass. A vast
number of studies have been dedicated to the effects of melittin’s regulation of apoptosis and to
the factors that induce apoptosis in various types of cancer such as breast, ovarian, prostate, lung.
The latest evidence indicates its potential as a therapeutic agent in the treatment of leukaemia.
The aim of our present study is to evaluate melittin’s ability to induce apoptosis in leukaemia cell
lines of different origin acute lymphoblastic leukaemia (CCRF-CEM) and chronic myelogenous
leukaemia (K-562). We demonstrated that melittin strongly reduced cell viability in both leukaemia
cell lines but not in physiological peripheral blood mononuclear cells (PMBCs). Subsequent estimated
parameters (mitochondrial membrane potential, Annexin V binding and Caspases 3/7 activity) clearly
demonstrated that melittin induced apoptosis in leukaemia cells. This is a very important step for
research into the development of new potential anti-leukaemia as well as anticancer therapies. Further
analyses on the molecular level have been also planned (analysis of proapoptotic genes expression
and DNA damages) for our next research project, which will also focus on melittin.
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