Dioksygenazy TET i O-GlcNAc transferaza w progresji nowotworów endometrium

Abstract

In many types of cancer, impaired expression and activity of TET proteins is found. TET proteins can interact with OGT, allowing it to be transported to chromatin, where it modifies histone proteins. Therefore, the OGT-TET complex may have a significant impact on the regulation of gene expression. Although all three TET proteins influence the recruitment of OGT to chromatin, it is suggested that the TET3 protein plays a key role in this process. The aim of the dissertation was to determine the relationship between the expression of TET and OGT genes and the progression of endometrial cancer, as well as the impact of the interactions between TET3 and OGT on the regulation of the expression of genes related to the EMT and the ability of endometrial cancer cells to invade and metastasize. The results of studies carried out on tissues collected from patients with endometrial cancer indicate that the levels of TET1 and TET2 transcripts, but not TET3, are reduced and correlated with the levels of 5-hmC. The results of studies conducted on endometrial cancer cell lines indicate that interactions between TET3 and OGT play a role in regulating the expression of genes involved in the EMT and genes influencing the ability of cells to invade and metastasize. However, the obtained results indicate the complex and cell-specific nature of the interactions between TET3 and OGT, and therefore further research is needed to determine the molecular context and identify additional factors that may influence the activity of these proteins.