http://hdl.handle.net/11089/1329 2026-08-09T22:30:29Z http://hdl.handle.net/11089/59015 Dataset for publication_Revealing the increased toxicity of PS-NPs after UV exposure towards PBMCs_funded by the National Science Centre, Poland (grant number 2023/07/X/ST5/01519)(data) Kisielewska, Aneta; Piwoński, Ireneusz; Pięta, Łukasz; Malek, Kamilla This study presents the effect of UV on the physicochemical changes of polystyrene nanoparticles (PS-NPs) with a size of 44 nm after 2, 4, and 8 h of illumination, along with its impact on white blood cells. It was found that UV illumination reduced the diameter of PS-NPs to 10.2 ± 0.4% and increased the zeta potential to 36 ± 3% after 8 h of illumination. Microscopy (SEM and AFM) measurements showed considerable changes in the shape and behaviour of PS-NPs, indicating reduced size and a high tendency to agglomerate when deposited on the substrate surface. Moreover, UV-Vis spectroscopy showed an increase in the intensity of the n-π* band, signifying oxidation of the nanomaterial. The chemical modifications significantly influenced the biological properties of the nanoparticles, altering the morphology of peripheral blood mononuclear cells (PBMCs) as indicated by changes in forward scatter (FSC) and side scatter (SSC) parameters. Additionally, there were modifications in reactive oxygen species (ROS) generation, which, in turn, affected cell viability. Prolonged UV exposure of nanoparticles can also lead to increase in the levels of intracellular chelatable iron ions (Fe²⁺), thereby indicating the induction of cell death associated with ferroptosis. The most pronounced alterations in these parameters were observed after incubating PBMCs with nanoparticles exposed to UV for 8 hours. Results from this study clearly demonstrate that the tested nanoplastic undergoes physicochemical changes upon exposure to UV, and that these induced changes strongly affect human peripheral blood mononuclear cells. 2024-01-01T00:00:00Z http://hdl.handle.net/11089/58873 Experimental datasets for the separation of voltammetric signals (dataset) Wypych, Monika; Jakubowska, Małgorzata; Świderski, Michał; Polit, Justyna; Koszelska, Kamila; Chwatko, Grażyna; Borowczyk, Kamila; Smarzewska, Sylwia This repository contains datasets supporting the evaluation of voltammetric signal-separation procedures for capsaicin and dihydrocapsaicin. The deposited files include datasets, an example baseline-corrected and smoothed voltammetric signal provided for workflow demonstration. 2026-07-01T00:00:00Z http://hdl.handle.net/11089/57734 Dataset for publication_Molecules 2025(30)4651_funded by National Science Center, Poland 2021/41/N/ST4/01425 Koszelska, Kamila; Świderski, Michał; Seroka, Jagoda; Guziejewski, Dariusz; Krzymiński, Paweł; Miniak-Górecka, Alicja; Ullah, Nabi; Smarzewska, Sylwia The study focused on evaluating the effect of electrode surface preparation on electrochemical measurements. In particular, the influence of different polishing protocols applied to carbon electrodes was investigated with respect to the electroactive surface area and the repeatability of the electrochemical response. The mechanical cleaning procedure of the solid disc electrode was systematically examined by testing various polishing motions and numbers of repetitions. The results demonstrated that the polishing method significantly affects both the electroactive surface area and measurement repeatability. The highest surface area and best repeatability were achieved using an “8-shaped” polishing motion. These findings confirm that electrode surface preparation plays a critical role in obtaining reliable and reproducible electroanalytical data. Dane badawcze powiązane z publikacją: Publikacja1. Świderski, M.; Seroka, J.; Guziejewski, D.; Krzymiński, P.; Miniak-Górecka, A.; Koszelska, K.; Ullah, N.; Smarzewska, S. Influence of Electrode Polishing Protocols, Potentiostat Models, and LOD Calculation Methods on the Electroanalytical Performance of SWV Measurements at Glassy Carbon Electrodes. Molecules 2025, 30, 4651. https://doi.org/10.3390/molecules30234651 In the file, measurement data obtained during voltammetric studies are provided. They present analyses carried out using different potentiostats, as well as various electrode surface renewal (cleaning/polishing) methods. 2025-01-01T00:00:00Z http://hdl.handle.net/11089/57733 Dataset for publication_Eng. Proc. 2023(48)65_funded by the National Science Center, Cracow, Poland (Grant no. 2021/41/N/ST4/01425) Machura, Gabriela; Seroka, Jagoda; Smarzewska, Sylwia; Koszelska, Kamila This study focused on the optimization of adrenaline electrografting onto a glassy carbon electrode using cyclic voltammetry in an acidic solution. A stable layer was obtained after multiple potential scans and characterized by square wave voltammetry. The results confirmed the successful formation of a stable adrenaline film on the electrode surface. Furthermore, the immobilized layer exhibited sensitivity to selected metal cations, as evidenced by a decrease in the adrenaline signal after exposure, indicating its potential application in indirect cation detection. The file contains measurement data obtained during voltammetric studies. It presents data collected during the deposition of adrenaline on the electrode surface and the subsequent optimization of this process. Additionally, it includes data aimed at verifying the sensitivity of the immobilized layer to the presence of Mg²⁺ and Ca²⁺ ions. 2023-01-01T00:00:00Z