http://hdl.handle.net/11089/37864 Dane badawcze zebrane w ramach projektów realizowanych na Wydziale Biologii i Ochrony Środowiska | Research data collected as part of projects carried out at the Faculty of Biology and Environmental Protection 2026-04-11T12:13:15Z http://hdl.handle.net/11089/57929 Wstępna ocena potencjału terapeutycznego miechunki pomidorowej (Physalis philadelphica) w kontekście przebudowy tkanek i gojenia ran (dataset) Liudvytska, Oleksandra Chronic wounds represent a growing problem in lifestyle diseases (e.g., atherosclerosis, diabetes). They are characterized, among other things, by disturbances in the mechanisms controlling the course of inflammatory processes, degeneration of the tissue surrounding the wound, scarring, and susceptibility to infections. Hard‑to‑heal wounds constitute a serious and burdensome issue for patients, as they significantly reduce their quality of life and require long-term, specialized therapy. The search for natural bioactive substances is one of the important directions in contemporary wound‑healing research. Natural compounds can serve as the basis for therapies that support all stages of healing, including modulation of the inflammatory response, fibroblast proliferation, and epithelial regeneration. They are often characterized by high biocompatibility and low cytotoxicity, making them very promising candidates for applications in wound‑healing support. The project focused on key aspects relevant to the potential use of extracts from the tomatillo (Physalis philadelphica) in wound‑healing support: anti‑inflammatory properties, effects on tissue remodeling, assessment of cytotoxicity risk, and metabolism by the skin microbiome. Four extracts were tested: from fruits (green – unripe, and yellow), leaves, and roots. The study employed dermal (fibroblast) and epithelial (keratinocyte) cell lines. Additionally, the effects of the extracts on proteins of the fibrinolytic system were evaluated, as this system plays an important regulatory role in tissue remodeling within the wound area. Spis plików: 1. Generowanie plazminy 2. IL- 6 3. IL- 8 4. Mikrobiota skóry 5. MMP3 6. MMP9 7. MTT 8. PLUA 9. PLUAR soluble 10. PLUAR 11. Test rany 2026-03-31T00:00:00Z http://hdl.handle.net/11089/57731 Opracowanie modelu badawczego in vitro do analiz wpływu stanu ketozy na angiogenezę nowotworową; Miniatura 8 (2024/08/X/NZ3/00599) (dataset) Soboska, Kamila As part of the Miniatura 8 research project, the effect of the ketosis state on tumour-associated angiogenesis was investigated. An in vitro model reflecting the conditions during ketogenic diet was developed, and the effects of glucose deprivation and ketone bodies were compared in tumour (TECs) and normal (NECs) endothelial cells. The studies were performed using the HMEC-1 cell line, in which a tumour endothelial cell model was generated under hypoxic conditions and in the presence of cancer cell-secreted pro-angiogenic factors. The expression of ketone body transporters and ketolytic enzymes was analysed at both the mRNA and protein levels in NECs and TECs, and their ability to metabolise ketone bodies was compared. In further experiments, the effect of ketone bodies on endothelial cell viability and the secretion of pro-angiogenic factors by colorectal cancer cell lines were evaluated. The dataset contains the results of the following tests: sqPCR from the analysis of the expression of markers confirming tumor endothelial cell model (TECs markers_sqPCR_RawData) and from the analysis of the expression of genes encoding ketolytic enzymes and the ketone body transporter (ketolysis_sqPCR_RawData), images of the results of the Western immunoblot analysis (WB scan), acetyl-CoA concentration in cells treated with ketone bodies (Acetyl-CoA conc_RawData), results of endothelial cell proliferation tests under conditions of variable glucose concentration (ECs prolif_glucose conc_RawData) and in conditions of simulated ketosis (ECs prolif_KBs conc_RawData) and results of the analysis of cytokine profiling secreted by tumor cells under simulated ketosis conditions (ProteomeProfiler) 2024-09-01T00:00:00Z http://hdl.handle.net/11089/57553 Untargeted metabolomic profiling for identifying systemic signatures of Helicobacter pylori infection in children (dataset) Gonciarz, Weronika Background. Infections caused by the Gram-negative bacterium Helicobacter pylori (H. pylori) can result in gastritis, gastric or duodenal ulcers, and gastric cancer in humans. Examining quantitative changes in soluble biomarkers linked to H. pylori infection offers a promising approach to monitor the infection's progression, inflammatory response, and systemic effects. Aim. This study aimed to identify specific metabolomic biomarkers in the sera from children infected with H. pylori. Materials and Methods. Sera from 32 H. pylori-infected children – Hp (+) and 32 uninfected children – Hp (-), under the pediatric gastroenterological medical care were used in this study. The H. pylori status in children was confirmed or excluded by gastroscopy, 13C urea breath testing, and the presence of serum anti-H. pylori IgG antibodies. Metabolomic profiling was performed using UPLC-QTOF/MS methods. Biomarkers significantly associated with H. pylori infection were identified using volcano plots and ROC analysis. Results. The study found 8 unique metabolites well differentiating the serum samples of children infected with H. pylori from children uninfected with these bacteria: carboxyethyl lysine - CEL (HMDB29447), gamma-Glutamylleucine (HMDB0011171), 13-HOTrE(y) hydroxylated and oxidized derivative of the omega-6 fatty acid arachidonic acid (HMDB0341541), 13-HODE (HMDB0004667), lauroylcarnitine (HMDB0002250), phosphatidylethanolamine (HMDB0060501), vitamin A (HMDB0000305) and 19_norandrosterone (HMDB0002697). These metabolites are associated with immune regulation, energy metabolism, lipid/fatty acid metabolism, lipid peroxidation, oxidative stress, and cell signaling, and correlate with the pathogenesis of H. pylori infection in humans. Conclusions. Serum metabolomic profiling combined with ROC analysis used in this study successfully differentiated H. pylori-infected from uninfected children. Further study is needed to link these selected metabolic biomarkers to the course of infection in children and to systemic effects of chronic infection. 2026-01-01T00:00:00Z http://hdl.handle.net/11089/57419 Aktywność biologiczna postbiotyków otrzymywanych z wykorzystaniem odpadów przemysłu rolno-spożywczego - MINIATURA 8 (2024/08/X/NZ9/00211) DATASET Nowak-Lange, Marta As part of the research project, the biological activity of postbiotics obtained using both commercially available probiotic microorganisms and isolates from fermented food products was evaluated. The microorganisms were cultivated on standard media as well as on media prepared from agri-food industry by-products, such as whey. A postbiotic extraction process was developed, and preliminary identification of metabolites present in the extracts and culture supernatants was performed. The antimicrobial activity of the postbiotics against eight skin pathogens was determined by establishing minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBCs/MFC) using the broth microdilution method. In addition, pathogen viability and the effects of postbiotics on biofilm formation and eradication were assessed. Their cytotoxicity toward eukaryotic cells was also evaluated. Furthermore, the antioxidant potential and anti-inflammatory properties of the obtained products were investigated. The conducted studies expanded the current knowledge on the relationship between postbiotic production conditions and their biological activity, including antimicrobial, antioxidant, and immunomodulatory properties, which may support the development of new, sustainable strategies for the production of cosmetic formulations. The contents of the files are described in detail in the ReadMe file. Some files have been blocked due to publication priority. 2024-07-01T00:00:00Z