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Interactions of the Gasotransmitters Contribute to Microvascular Tone (Dys)regulation in the Preterm Neonate

dc.contributor.authorDyson, Rebecca M.
dc.contributor.authorPalliser, Hannah K.
dc.contributor.authorLatter, Joanna L.
dc.contributor.authorKelly, Megan A.
dc.contributor.authorChwatko, Grażyna
dc.contributor.authorGlowacki, Rafal
dc.contributor.authorWright, Ian M. R.
dc.date.accessioned2015-06-23T12:15:18Z
dc.date.available2015-06-23T12:15:18Z
dc.date.issued2015-03-25
dc.identifier.urihttp://hdl.handle.net/11089/10077
dc.description.abstractBackground & Aims Hydrogen sulphide (H2S), nitric oxide (NO), and carbon monoxide (CO) are involved in transitional microvascular tone dysregulation in the preterm infant; however there is conflicting evidence on the interaction of these gasotransmitters, and their overall contribution to the microcirculation in newborns is not known. The aim of this study was to measure the levels of all 3 gasotransmitters, characterise their interrelationships and elucidate their combined effects on microvascular blood flow. Methods 90 preterm neonates were studied at 24h postnatal age. Microvascular studies were performed by laser Doppler. Arterial COHb levels (a measure of CO) were determined through co-oximetry. NO was measured as nitrate and nitrite in urine. H2S was measured as thiosulphate by liquid chromatography. Relationships between levels of the gasotransmitters and microvascular blood flow were assessed through partial correlation controlling for the influence of gestational age. Structural equation modelling was used to examine the combination of these effects on microvascular blood flow and derive a theoretical model of their interactions. Results No relationship was observed between NO and CO (p = 0.18, r = 0.18). A positive relationship between NO and H2S (p = 0.008, r = 0.28) and an inverse relationship between CO and H2S (p = 0.01, r = -0.33) exists. Structural equation modelling was used to examine the combination of these effects on microvascular blood flow. The model with the best fit is presented. Conclusions The relationships between NO and H2S, and CO and H2S may be of importance in the preterm newborn, particularly as NO levels in males are associated with higher H2S levels and higher microvascular blood flow and CO in females appears to convey protection against vascular dysregulation. Here we present a theoretical model of these interactions and their overall effects on microvascular flow in the preterm newborn, upon which future mechanistic studies may be based.pl_PL
dc.description.sponsorshipThe authors would like to acknowledge the parents of the neonates enrolled in the 2CANS study for their participation, the staff of the Kaleidoscope Neonatal Intensive Care Unit at the John Hunter Children’s Hospital, and Kimberly-Clark Australia for providing the diapers used in this studypl_PL
dc.language.isoenpl_PL
dc.publisherPublic Library Of Sciencepl_PL
dc.relation.ispartofseriesPLOS ONE;
dc.rightsUznanie autorstwa 3.0 Polska*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/pl/*
dc.titleInteractions of the Gasotransmitters Contribute to Microvascular Tone (Dys)regulation in the Preterm Neonatepl_PL
dc.typeArticlepl_PL
dc.contributor.authorAffiliationDyson Rebecca M., Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, School of Medicine and Public Health, University of Newcastle, Illawarra Health and Medical Research Institute and Graduate School of Medicine, University of Wollongongpl_PL
dc.contributor.authorAffiliationPalliser Hannah K., Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, School of Biomedical Sciences and Pharmacy, University of Newcastlepl_PL
dc.contributor.authorAffiliationLatter Joanna L., Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, School of Medicine and Public Health, University of Newcastlepl_PL
dc.contributor.authorAffiliationKelly, Megan A. Illawarra Health and Medical Research Institute and Graduate School of Medicine, University of Wollongongpl_PL
dc.contributor.authorAffiliationChwatko Grazyna, University of Lodz, Department of Environmental Chemistry, Faculty of Chemistrypl_PL
dc.contributor.authorAffiliationGlowacki, Rafal, University of Lodz, Department of Environmental Chemistry, Faculty of Chemistrypl_PL
dc.contributor.authorAffiliationWright, Ian M. R. Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, School of Medicine and Public Health, University of Newcastle, Illawarra Health and Medical Research Institute and Graduate School of Medicine, University of Wollongong, Kaleidoscope Neonatal Intensive Care Unit, John Hunter Children’s Hospitapl_PL
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dc.contributor.authorEmailiwright@uow.edu.aupl_PL


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