The effect of EDTA and EDDS on lead uptake and localization in hydroponically grown Pisum sativum L.
Streszczenie
Pisum sativum plants were treated for 3 days
with an aqueous solution of 100 lM Pb(NO3)2 or with a
mixture of lead nitrate and ethylenediaminetetraacetic acid
(EDTA) or [S,S]-ethylenediaminedisuccinic acid (EDDS)
at equimolar concentrations. Lead decline from the incubation
media and its accumulation and localization at the
morphological and ultrastructural levels as well as plant
growth parameters (root growth, root and shoot dry weight)
were estimated after 1 and 3 days of treatment. The tested
chelators, especially EDTA, significantly diminished Pb
uptake by plants as compared to the lead nitrate-treated
material. Simultaneously, EDTA significantly enhanced Pb
translocation from roots to shoots. In the presence of both
chelates, plant growth parameters remained considerably
higher than in the case of uncomplexed Pb. Considerable
differences between the tested chelators were visible in Pb
localization both at the morphological and ultrastructural
level. In Pb?EDTA-treated roots, lead was mainly located
in the apical parts, while in Pb?EDDS-exposed material
Pb was evenly distributed along the whole root length.
Transmission electron microscopy and EDS analysis
revealed that in meristematic cells of the roots incubated in
Pb?EDTA, large electron-dense lead deposits were located
in vacuoles and small granules were rarely noticed in cell
walls or cytoplasm, while after Pb?EDDS treatment metal
deposits were restricted to the border between plasmalemma
and cell wall. Such results imply different ways of
transport of those complexed Pb forms.
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