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Antioxidant action of SMe1EC2, the low-basicity derivative of the pyridoindole stobadine, in cell free chemical models and at cellular level

dc.contributor.authorBalcerczyk, Aneta
dc.contributor.authorBartosz, Grzegorz
dc.contributor.authorDrzewinska, Joanna
dc.contributor.authorPiotrowski, Łukasz
dc.contributor.authorPulaski, Lukasz
dc.contributor.authorStefek, Milan
dc.date.accessioned2016-04-07T11:55:14Z
dc.date.available2016-04-07T11:55:14Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/11089/17744
dc.description.abstractThe aim of the study was to evaluate the antioxidant action of SMe1EC2, the structural analogue of the hexahydropyridoindole antioxidant stobadine. The antiradical activity of SMe1EC2 was found to be higher when compared to stobadine, as determined both in cell-free model systems of AAPH-induced oxidation of dihydrorhodamine 123 and 2´,7´-dichloro-dihydrofluorescein diacetate, and in the cellular system of stimulated macrophages RAW264.7. Analysis of proliferation of HUVEC and HUVEC-ST cells revealed absence of cytotoxic effect of SMe1EC2 at concentrations below 100 μM. The antioxidant activity of SMe1EC2, superior to the parent drug stobadine, is accounted for by both the higher intrinsic free radical scavenging action and by the better bioavailability of the low-basicity SMe1EC2 relative to the high-basicity stobadine.pl_PL
dc.description.sponsorshipFinancial support by the grants VEGA 2/0067/11 and COST CM1001 action is gratefully acknowledgedpl_PL
dc.language.isoenpl_PL
dc.publisherDe Gruyter Openpl_PL
dc.relation.ispartofseriesInterdisciplinary Toxicology;1
dc.rightsUznanie autorstwa 3.0 Polska*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/pl/*
dc.subjectantioxidantpl_PL
dc.subjecthexahydropyridoindolepl_PL
dc.subjectSMe1EC2pl_PL
dc.subjectstobadinepl_PL
dc.subjectoxidative stresspl_PL
dc.titleAntioxidant action of SMe1EC2, the low-basicity derivative of the pyridoindole stobadine, in cell free chemical models and at cellular levelpl_PL
dc.typeArticlepl_PL
dc.page.number27–32pl_PL
dc.contributor.authorAffiliationUniversity of Lodz, Department of Molecular Biophysicspl_PL
dc.contributor.authorAffiliationUniversity of Rzeszow, Department of Biochemistry and Cell Biologypl_PL
dc.contributor.authorAffiliationCenter of Medical Biology, Laboratory of Transcriptional Regulationpl_PL
dc.contributor.authorAffiliationSlovak Academy of Sciences, Institute of Experimental Pharmacology and Toxicologypl_PL
dc.identifier.eissn1337-9569
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dc.contributor.authorEmailmilan.stefek@savba.skpl_PL
dc.identifier.doi10.2478/intox-2014-0005
dc.relation.volume7pl_PL


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