Pokaż uproszczony rekord

dc.contributor.authorCiesielska, Anita
dc.contributor.authorStączek, Paweł
dc.date.accessioned2021-09-14T09:30:52Z
dc.date.available2021-09-14T09:30:52Z
dc.date.issued2020
dc.identifier.citationCite this article Ciesielska, A., Stączek, P. A new molecular marker for species-specific identification of Microsporum canis. Braz J Microbiol 51, 1505–1508 (2020). https://doi.org/10.1007/s42770-020-00340-ypl_PL
dc.identifier.issn1517-8382
dc.identifier.urihttp://hdl.handle.net/11089/39053
dc.description.abstractSpecies identification of dermatophytes by conventional mycological methods based on macro- and microscopy analysis is time-consuming and has a lot of limitations such as slow fungal growth or low specificity. Thus, there is a need for the development of molecular methods that would provide reliable and prompt identification of this group of medically important fungi. The are many reports in the literature concerning PCR identification of dermatophyte species, but still, there are not many PCR assays for the separate detection of members of the genera Microsporum, especially Microsporum canis (zoophilic species) and Microsporum audouinii (anthropophilic species). The correct distinction of these species is important to determine the source of infection to implement the appropriate action to eliminate the path of infection transmission. In this paper, we present such a PCR-based method targeting velB gene that uses a set of two primers—Mc-VelB-F (5′-CTTCCCCACCCGCAACATC-3′) and Mc-VelB-R (5′-TGTGGCTGCACCTGAGAGTGG-3′). The amplified fragment is specific due to the presence of (CAGCAC)8 microsatellite sequence only in the velB gene of M. canis. DNA from 153 fungal samples was used in PCR assay followed by electrophoretic analysis. The specificity of the designed set of primers was also confirmed using the online BLAST-Primer tool. The positive results were observed only in the case of M. canis isolates, and no positive results were obtained neither for other dermatophytes and non-dermatophyte fungi nor for other Eukaryotes, including the human genome sequence, as well as the representatives of bacterial and viral taxa. The developed PCR assay using the proposed Mc-VelB-F and Mc-velB-R primers can be included in the algorithm of M. canis detection in animals and humans.pl_PL
dc.language.isoenpl_PL
dc.publisherSpringer Naturepl_PL
dc.relation.ispartofseriesBrazilian Journal of Microbiology;51
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMicrosporum canispl_PL
dc.subjectPCRpl_PL
dc.subjectMolecular identificationpl_PL
dc.subjectMolecular markerpl_PL
dc.titleA new molecular marker for species-specific identification of Microsporum canispl_PL
dc.typeArticlepl_PL
dc.page.number1505–1508pl_PL
dc.contributor.authorAffiliationDepartment of Microbial Genetics, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Microbial Genetics, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Polandpl_PL
dc.identifier.eissn1678-4405
dc.referencesRezaei-Matehkolaei A, Makimura K, Sybren de Hoog G, Shidfar MR, Satoh K, Najafzadeh MJ, Mirhendi H (2012) Multilocus differentiation of the related dermatophytes Microsporum canis, Microsporum ferrugineum and Microsporum audouinii. J Med Microbiol 61:57–63. https://doi.org/10.1099/jmm.0.036541-0pl_PL
dc.referencesDobrowolska A, Stączek P, Kaszuba A, Kozłowska M (2006) PCR-RFLP analysis of the dermatophytes isolated from patients in Central Poland. J Dermatol Sci 42:71–74. https://doi.org/10.1016/j.jdermsci.2006.01.001pl_PL
dc.referencesBrillowska-Dąbrowska A, MichaŁek E, Saunte DML, Sogaard Nielsen S, Arendrup MC (2013) PCR test for Microsporum canis identification. Med Mycol 51:576–579. https://doi.org/10.3109/13693786.2012.755741pl_PL
dc.referencesBrillowska-Dąbrowska A, Wierkowska A, Lindhardt Saunte DM, Arendrup MC (2010) Diagnostic PCR tests for Microsporum audouinii, M. canis and Trichophyton infections. Med Mycol 48:486–490. https://doi.org/10.3109/13693780903312454pl_PL
dc.referencesKobylak N, Bykowska B, Kurzyk E, Nowicki R, Brillowska-Dąbrowska A (2016) PCR and real-time PCR approaches to the identification of Arthroderma otae species Microsporum canis and Microsporum audouinii/Microsporum ferrugineum. J Eur Acad Dermatol Venereol 30:1819–1822. https://doi.org/10.1111/jdv.13681pl_PL
dc.referencesBayram Ö, Braus GH (2012) Coordination of secondary metabolism and development in fungi: the velvet family of regulatory proteins. FEMS Microbiol Rev 36:1–24. https://doi.org/10.1111/j.1574-6976.2011.00285.xpl_PL
dc.referencesJackson CJ, Barton RC, Evans EGV (1999) Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions. J Clin Microbiol 37:931–936pl_PL
dc.referencesKoressaar T, Remm M (2007) Enhancements and modifications of primer design program Primer3. Bioinformatics 23:1289–1291pl_PL
dc.contributor.authorEmailanita.ciesielska@biol.uni.lodz.plpl_PL
dc.identifier.doihttps://doi.org/10.1007/s42770-020-00340-y
dc.disciplinenauki biologicznepl_PL


Pliki tej pozycji

Thumbnail
Thumbnail

Pozycja umieszczona jest w następujących kolekcjach

Pokaż uproszczony rekord

Uznanie autorstwa 4.0 Międzynarodowe
Poza zaznaczonymi wyjątkami, licencja tej pozycji opisana jest jako Uznanie autorstwa 4.0 Międzynarodowe