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dc.contributor.authorTutka, Klaudia
dc.contributor.authorŻychowska, Magdalena
dc.contributor.authorReich, Adam
dc.contributor.authorŻaczek, Anna
dc.contributor.authorMaternia-Dudzik, Karolina
dc.contributor.authorPawełczyk, Jakub
dc.contributor.authorStrapagiel, Dominik
dc.contributor.authorLach, Jakub
dc.date.accessioned2023-10-27T05:01:58Z
dc.date.available2023-10-27T05:01:58Z
dc.date.issued2023
dc.identifier.citationTutka, K.; Zychowska, M.; ˙ Zaczek, A.; Maternia-Dudzik, K.; ˙ Pawełczyk, J.; Strapagiel, D.; Lach, J.; Reich, A. Skin Microbiome in Prurigo Nodularis. Int. J. Mol. Sci. 2023, 24, 7675. https://doi.org/10.3390/ ijms24087675pl_PL
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/11089/48203
dc.description.abstractPrurigo nodularis (PN) is a chronic condition characterized by the presence of nodular lesions accompanied by intense pruritus. The disease has been linked to several infectious factors, but data on the direct presence of microorganisms in the lesions of PN are scarce. The aim of this study was to evaluate the diversity and composition of the bacterial microbiome in PN lesions by targeting the region V3-V4 of 16S rRNA. Skin swabs were obtained from active nodules in 24 patients with PN, inflammatory patches of 14 patients with atopic dermatitis (AD) and corresponding skin areas of 9 healthy volunteers (HV). After DNA extraction, the V3-V4 region of the bacterial 16S rRNA gene was amplified. Sequencing was performed using the Illumina platform on the MiSeq instrument. Operational taxonomic units (OTU) were identified. The identification of taxa was carried out using the Silva v.138 database. There was no statistically significant difference in the alpha-diversity (intra-sample diversity) between the PN, AD and HV groups. The beta-diversity (inter-sample diversity) showed statistically significant differences between the three groups on a global level and in paired analyses. Staphylococcus was significantly more abundant in samples from PN and AD patients than in controls. The difference was maintained across all taxonomic levels. The PN microbiome is highly similar to that of AD. It remains unclear whether the disturbed composition of the microbiome and the domination of Staphylococcus in PN lesions may be the trigger factor of pruritus and lead to the development of cutaneous changes or is a secondary phenomenon. Our preliminary results support the theory that the composition of the skin microbiome in PN is altered and justify further research on the role of the microbiome in this debilitating condition.pl_PL
dc.description.sponsorshipThis research was supported by the mini-Dgrant provided by the Polish Dermatological Society to Dr. Magdalena Zychowska (1/2020).pl_PL
dc.language.isoenpl_PL
dc.publisherMDPIpl_PL
dc.relation.ispartofseriesInternational Journal of Molecular Sciences;24, 7675
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectmicrobiomepl_PL
dc.subjectmicrobiotapl_PL
dc.subjectprurigo nodularispl_PL
dc.subjectbacteriapl_PL
dc.subject16S rRNApl_PL
dc.subjectDNA sequencingpl_PL
dc.titleSkin Microbiome in Prurigo Nodularispl_PL
dc.typeArticlepl_PL
dc.page.number18pl_PL
dc.contributor.authorAffiliationDepartment of Dermatology, Institute of Medical Sciences, Medical College of Rzeszow University, 35-055 Rzeszów, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Dermatology, Institute of Medical Sciences, Medical College of Rzeszow University, 35-055 Rzeszów, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Dermatology, Institute of Medical Sciences, Medical College of Rzeszow University, 35-055 Rzeszów, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Microbiology, Institute of Medical Sciences, Medical College of Rzeszow University, 35-055 Rzeszów, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Microbiology, Institute of Medical Sciences, Medical College of Rzeszow University, 35-055 Rzeszów, Polandpl_PL
dc.contributor.authorAffiliationBiobank Laboratory, Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-235 Łódź, Polandpl_PL
dc.contributor.authorAffiliationBiobank Laboratory, Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-235 Łódź, Polandpl_PL
dc.identifier.eissn1422-0067
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dc.identifier.doi10.3390/ ijms24087675
dc.disciplinenauki biologicznepl_PL


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