| dc.contributor.author | Soboska, Kamila | |
| dc.date.accessioned | 2026-03-18T13:25:27Z | |
| dc.date.available | 2026-03-18T13:25:27Z | |
| dc.date.issued | 2024-09 | |
| dc.identifier.uri | http://hdl.handle.net/11089/57731 | |
| dc.description | The dataset contains the results of the following tests: sqPCR from the analysis of the expression of markers confirming tumor endothelial cell model (TECs markers_sqPCR_RawData) and from the analysis of the expression of genes encoding ketolytic enzymes and the ketone body transporter (ketolysis_sqPCR_RawData), images of the results of the Western immunoblot analysis (WB scan), acetyl-CoA concentration in cells treated with ketone bodies (Acetyl-CoA conc_RawData), results of endothelial cell proliferation tests under conditions of variable glucose concentration (ECs prolif_glucose conc_RawData) and in conditions of simulated ketosis (ECs prolif_KBs conc_RawData) and results of the analysis of cytokine profiling secreted by tumor cells under simulated ketosis conditions (ProteomeProfiler) | pl_PL |
| dc.description.abstract | As part of the Miniatura 8 research project, the effect of the ketosis state on tumour-associated angiogenesis was investigated. An in vitro model reflecting the conditions during ketogenic diet was developed, and the effects of glucose deprivation and ketone bodies were compared in tumour (TECs) and normal (NECs) endothelial cells. The studies were performed using the HMEC-1 cell line, in which a tumour endothelial cell model was generated under hypoxic conditions and in the presence of cancer cell-secreted pro-angiogenic factors.
The expression of ketone body transporters and ketolytic enzymes was analysed at both the mRNA and protein levels in NECs and TECs, and their ability to metabolise ketone bodies was compared. In further experiments, the effect of ketone bodies on endothelial cell viability and the secretion of pro-angiogenic factors by colorectal cancer cell lines were evaluated. | pl_PL |
| dc.description.sponsorship | Badania zostały sfinansowane przez Narodowe Centrum Nauki (NCN) w ramach konkursu Miniatura 8 (2024/08/X/NZ3/00599) Opracowanie modelu badawczego in vitro do analiz wpływu stanu ketozy na angiogenezę nowotworową. | pl_PL |
| dc.language.iso | en | pl_PL |
| dc.subject | ketogenic diet | pl_PL |
| dc.subject | colorectal cancer | pl_PL |
| dc.subject | angiogenesis | pl_PL |
| dc.title | Opracowanie modelu badawczego in vitro do analiz wpływu stanu ketozy na angiogenezę nowotworową; Miniatura 8 (2024/08/X/NZ3/00599) (dataset) | pl_PL |
| dc.type | Dataset | pl_PL |
| dc.contributor.authorAffiliation | Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz | pl_PL |
| dc.discipline | nauki biologiczne | pl_PL |
