Isolation and characterization of 8 microsatellite loci for the ‘‘killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)
Streszczenie
Dikerogammarus villosus is a freshwater
amphipod of the Ponto-Caspian origin recognized as one of
the 100 worst alien species in Europe, having negative
impact on biodiversity and functioning of the invaded
aquatic ecosystems. The species has a wide ecophysiological
tolerance and during the last 20 years it has rapidly
spread throughout European inland waters. In consequence,
it presents a major conservation management problem. We
describe eight polymorphic microsatellite loci developed
for D. villosus by combining a biotin-enrichment protocol
and new generation 454GS-FLX Titanium pyrosequencing
technology. When genotyped in 64 individuals from two
locations, the loci exhibited a mean diversity of 4.87 alleles
per locus (2–13). The mean observed and expected heterozygosities
were, respectively, 0.439 (0.091–0.844) and
0.468 (0.089–0.843). Gametic disequilibrium was not
detected for any pair of loci. The microsatellite markers
will be a valuable tool in assessing the demographic processes
associated with invasion of the killer shrimp from a
genetic point of view.
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