Wpływ konserwacji krwi na zawartość kwasów nukleinowych i aktywności enzymów nukleolitycznych w limfocytach ludzkiej krwi obwodowej

Abstract

The lymphocytes from human peripheral blood of healthy donors stored from 4°C to 6°C in three different conserving fluids of the following composition: 1) ACDf.B: 0.045 M trisodium citrate, 0.025 M citric acid, 0.035 M glucose, pH = 4.9; 2) CPD: 0.088 M trisodium citrate, 0.015 M citric acid 0.139 M glucose, 0.158 M sodium phosphate, pH - 5.63; 3) CPD with the addition of adeni ne and adenosine, were used as the material for investigations. The conservation time of blood was 0, 7, 14, 21 days for ACDf.B; 0, 7, 14, 21, 28 days for CPD and 0, 7, 14, 21, 28, 42 days for CPD with adenine and adenosine. The contents of RNA and DNA were determined by spectrophotometry method of Markov and Tsanev, the activity of ribonuclease by the method of Frih-Niggemayer-Reddi, deoxyribonuclease by the method of Rotherham and acid phosphatase by King-Armstrong’s method. Some changes were found in the DNA and RNA contents and in the activity of Investigated enzymes, depending on the time and conditions of blood conservation. These changes resemble the processes observed in mitogen stimulated lymphoyctes.