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RT-PCR Analysis of TOPBP1 Gene Expression in Hereditary Breast Cancer

dc.contributor.authorForma, Ewaen
dc.contributor.authorBernaciak, Magdalenaen
dc.contributor.authorRomanowicz-Makowska, Hannaen
dc.contributor.authorBryś, Magdalenaen
dc.date.accessioned2015-06-17T08:35:25Z
dc.date.available2015-06-17T08:35:25Z
dc.date.issued2011-03-04en
dc.identifier.issn1730-2366
dc.identifier.urihttp://hdl.handle.net/11089/9868
dc.description.abstractHereditary predisposition to breast cancer determined in large part by loss of function mutations in one of two genes BRCA1 and BRCA2. Besides BRCA1 and BRCA2 other genes are also likely to be involved in hereditary predisposition to breast cancer. TopBP1 protein is involved in DNA replication, DNA damage checkpoint response and transcriptional regulation. Expression of TopBP1 gene at the mRNA level was analyzed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) in 94 samples of hereditary breast cancer. Analysis of TopBP1 mRNA level showed that expression of TopBP1 is significantly downregulated in poorly differentiated breast cancer (grade III according Bloom-Richardson system (P<0.05).en
dc.publisherWydawnictwo Uniwersytetu Łódzkiegoen
dc.relation.ispartofseriesFolia Biologica et Oecologica;6en
dc.rightsThis content is open access.en
dc.subjectTopBP1en
dc.subjectgene expressionen
dc.subjectRT-PCRen
dc.subjecthereditary breast canceren
dc.titleRT-PCR Analysis of TOPBP1 Gene Expression in Hereditary Breast Canceren
dc.page.number49-59en
dc.contributor.authorAffiliationForma Ewa - Department of Cytobiochemistry, University of Łódź, Pomorska 141/143, 90-236 Łódź, Polanden
dc.contributor.authorAffiliationBernaciak Magdalena - Department of Cytobiochemistry, University of Łódź, Pomorska 141/143, 90-236 Łódź, Polanden
dc.contributor.authorAffiliationRomanowicz-Makowska Hanna - Department of Clinical Pathomorphology, Polish Mother's Memorial Hospital, Research Institute, Rzgowska 281/289, 93-338 Łódź, Polanden
dc.contributor.authorAffiliationBryś Magdalena - Department of Cytobiochemistry, University of Łódź, Pomorska 141/143, 90-236 Łódź, Polanden
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dc.identifier.doi10.2478/v10107-009-0006-1en


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